gDNA Q-PCR for clinical monitoring of CML
نویسندگان
چکیده
منابع مشابه
Optimizing fusion transcript monitoring in CML.
The recently published recommendations for the harmonization of methodology for BCR-ABL fusion transcript monitoring in chronic myeloid leukemia (CML) patients responding to treatment with tyrosine kinase inhibitors are timely and pertinent for all laboratories undertaking such testing.1 The authors provide practical guidelines for laboratories to help optimize their testing and reporting proce...
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BACKGROUND Although imatinib is highly effective in chronic myeloid leukemia (CML), drug-resistance may occur. Therefore, monitoring of minimal residual disease (MRD) during treatment with imatinib is important. However, most MRD-parameters are expensive and require special technology. We determined the value of histamine as MRD-marker in CML. PATIENTS AND METHODS Histamine levels were measur...
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UNLABELLED Chronic myelogenous leukemia (CML) is characterized by the Philadelphia chromosome and the BCR-ABL fusion gene that encodes an abnormal tyrosine kinase. Development of specific tyrosine kinase inhibitors completely changed the management of these patients. MATERIALS AND METHODS Between April 2008 and July 2012, at the Molecular Biology Laboratory, University of Medicine and Pharmac...
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Imatinib mesylate (IM) is the first line therapy against Chronic Myeloid Leukemia, effectively prolonging overall survival. Because discontinuation of treatment is associated with relapse, IM is required indefinitely to maintain operational cure. To assess minimal residual disease, cytogenetic analysis is insensitive in a high background of normal lymphocytes. The qRT-PCR provides highly sensit...
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The advances in the treatment strategies in CML epitomize the concept of personalized medicine. These rapid developments towards cure of CML are acutely dependent on our ability to monitor therapeutic response to the drugs and fine-tune the therapy to the challenges posed by drug resistance from development of target site mutations. The fundamental requirement for success of targeted therapy in...
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ژورنال
عنوان ژورنال: Cell Cycle
سال: 2015
ISSN: 1538-4101,1551-4005
DOI: 10.1080/15384101.2015.1006053